Mefuta e 15 ea Kotsi e Phahameng ea Papillomavirus ea Motho E6/E7 Gene mRNA
Lebitso la sehlahisoa
HWTS-CC005A-15 Mefuta ea Kotsi e Phahameng ea Papillomavirus ea Motho E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
Epidemiology
Kankere ea molomo oa popelo ke e 'ngoe ea mefuta e atileng haholo ea mofetše oa basali lefatšeng ka bophara, 'me ho hlaha ha eona ho amana haufi-ufi le human papillomaviruses (HPV), empa ke karolo e nyane feela ea tšoaetso ea HPV e ka fetohang mofetše.HPV e kotsing e kholo e tšoaetsa lisele tsa epithelial tsa mokokotlo 'me e hlahisa li-oncoprotein tse peli, E6 le E7.Protheine ena e ka ama mefuta e fapaneng ea liprotheine tsa cellular (tse kang tumor suppressor proteins pRB le p53), lelefatsa potoloho ea sele, ea ama DNA synthesis le genome botsitso, le ho kena-kenana le likarabo tsa antiviral le antitumor.
Channel
| Channel | Karolo | Genotype e lekoa |
| FAM | HPV Reaction Buffer 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | Mofuta oa motho oa β-actin | |
| FAM | HPV Reaction Buffer 2 | HPV 18,39,45,53,56,59,66,68 |
| VIC/HEX | Mofuta oa INS oa motho |
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | cervical exfoliated cell |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Likopi / mL |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Momollo o lokela ho etsoa ho latela litaelo tsa tšebeliso. .Molumo o khothaletsoang oa elution ke 50μL.Haeba sampole e sa chesoa ka botlalo, e khutlisetse mohatong oa 4 bakeng sa ho cheka hape.Ebe u leka ho latela litaelo tsa tšebeliso.
Sehlahisoa sa ho ntša se khothalelitsoeng: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso e tiileng (Mohatong oa 5, habeli khatello ea tharollo ea ts'ebetso ea DNaseI, ke hore, nka 20μL ea RNase-Free DNaseI (1500U) stock solution ka har'a tube e ncha ea RNase-Free centrifuge, eketsa 60μL ea RDD buffer, 'me u kopanye ka bonolo).Molumo o khothaletsoang oa elution ke 60μL.Haeba sampole e sa chesoa ka botlalo, e khutlisetse mohatong oa 5 bakeng sa ho cheka hape.Ebe u leka ho latela litaelo tsa tšebeliso.
