Mefuta e 28 ea Kotsi e Phahameng ea Papilloma Virus ea Motho (Typing ea 16/18) Nucleic Acid
Lebitso la sehlahisoa
HWTS-CC006A-28 Mefuta ea Kotsi e Phahameng ea Papilloma Virus ea Motho (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kankere ea popelo ke e 'ngoe ea lihlahala tse kotsi tse atileng ka ho fetesisa tsa pampitšana ea pelehi ea mosali.Liphuputso li bontšitse hore tšoaetso ea HPV e phehellang le mafu a mangata ke e 'ngoe ea lisosa tse ka sehloohong tsa mofetše oa molomo oa popelo.Hajoale, liphekolo tse lemohuoeng tse sebetsang hantle li ntse li haella bakeng sa mofetše oa popelo o bakoang ke HPV, kahoo ho sibolloa kapele le thibelo ea tšoaetso ea molomo oa popelo e bakoang ke HPV ke senotlolo sa ho thibela mofetše oa popelo.Ho bohlokoa haholo ho theha tlhahlobo e bonolo, e tobileng le e potlakileng ea tlhahlobo ea etiology bakeng sa tlhahlobo ea bongaka le kalafo ea mofetše oa popelo.
Channel
Reaction Mix | Channel | Mofuta |
PCR-Mix1 | FAM | 18 |
VIC(HEX) | 16 | |
ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
CY5 | Taolo ea ka hare | |
PCR-Mix2 | FAM | 6, 11, 54, 83 |
VIC(HEX) | 26, 44, 61, 81 | |
ROX | 40, 42, 43, 53, 73, 82 | |
CY5 | Taolo ea ka hare |
Tekheniki Parameters
Polokelo | Mokelikeli: ≤-18℃ |
Shelf-bophelo | Likhoeli tse 12 |
Mofuta oa Mohlala | cervical exfoliated cell |
Ct | ≤28 |
CV | ≤5.0% |
LoD | 300Likopi / mL |
Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®480 Real-Nako PCR Systems LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Li-reagents tse khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48), le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Khetho ea 2.
Li-reagents tse khothalelitsoeng tsa ho ntša: Nucleic Acid Extraction kapa Purification Kit(YDP315) ke Tiangen Biotech (Beijing) Co.,Ltd.