Herpes Simplex Virus Type 2 Nucleic Acid

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho lemoha boleng ba herpes simplex kokoana-hloko ea mofuta oa 2 nucleic acid ho banna ba urethral swab le lisampole tsa cervical swab.

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HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)

Tšebeliso e reriloeng

Setsi sena se sebelisetsoa ho lemoha boleng ba herpes simplex kokoana-hloko ea mofuta oa 2 nucleic acid ho banna ba urethral swab le lisampole tsa cervical swab.

Epidemiology

Herpes Simplex Virus Type 2 (HSV2) ke kokoana-hloko e selikalikoe e entsoeng ka tegument, capsid, core, le enfelopo, 'me e na le DNA ea mela e habeli.kokoana-hloko ea Herpes e ka kena 'meleng ka ho kopana ka ho toba kapa ho kopanela liphate le letlalo le li-membrane tsa mucous,' me e arotsoe ka sehloohong le se tloaelehileng.Tšoaetso ea kokoana-hloko e bakoa haholo ke HSV2, bakuli ba banna ba bonahatsoa e le liso tsa botona, 'me bakuli ba basali ba bonahatsoa e le liso tsa molomo, vulvar le botšehali.Tšoaetso ea pele ea kokoana-hloko ea genital herpes hangata ke ts'oaetso e feteletseng, ntle le likokoana-hloko tse seng kae tsa lehae tse nang le lera kapa letlalo, tseo bongata ba tsona li se nang matšoao a hlakileng a kliniki.Genital herpes tšoaetso e na le litšobotsi tsa bophelo bohle ba kokoana-hloko e jereng le ho ipheta habonolo, 'me bakuli ka bobeli le bajari ke mohloli oa tšoaetso ea lefu lena.Chaena, sekhahla sa serological positive sa HSV2 se ka ba 10.80% ho isa ho 23.56%.Mokhahlelo oa tšoaetso ea HSV2 o ka aroloa ka ts'oaetso ea mantlha le ts'oaetso ea khafetsa, 'me hoo e ka bang 60% ea bakuli ba nang le tšoaetso ea HSV2 ba khutlela mokhoeng oo.

Epidemiology

FAM: Herpes Simplex Virus Type 2 (HSV2)·

VIC(HEX): Taolo ea ka hare

PCR Amplification Conditions Setting

Mohato	Lipotoloho	Mocheso	Nako	BokellaFluorescentSli-ignalskapa eseng joalo
1	1 potoloho	50 ℃	5 mets	No
2	1 potoloho	95℃	10metsotso	No
3	40 Lipotoloho	95℃	15secs	No
4	40 Lipotoloho	58℃	31secs	Ee

Tekheniki Parameters

Polokelo
Mokelikeli	≤-18℃ Lefifing
Shelf-bophelo	Likhoeli tse 12
Mofuta oa Mohlala	Sekoti sa molomo oa popelo sa mosali, Sesepa sa botona
Ct	≤38
CV	≤5.0%
LoD	50Likopi/karabelo
Tse khethehileng	Ha ho na cross-reactivity le likokoana-hloko tse ling tsa STD, tse kang Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium joalo-joalo.
Lisebelisoa tse sebetsang	E ka tsamaisana le lisebelisoa tsa PCR tsa fluorescent tse fumanehang 'marakeng. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System.