Motho BRAF Gene V600E Phetoho
Lebitso la sehlahisoa
HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit(Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Ho fumanoe mefuta e fetang 30 ea liphetoho tsa BRAF, eo hoo e ka bang 90% e fumanehang ho exon 15, moo phetoho ea V600E e nkoang e le phetoho e tloaelehileng haholo, ke hore, thymine(T) sebakeng sa 1799 ho exon 15 e fetoletsoeng ho adenine (A), e bakang ho nkeloa sebaka ha valine (V) sebakeng sa 600 ka glutamic acid (E) sehlahisoa sa protheine.Liphetoho tsa BRAF li atisa ho fumanoa lihlahala tse kotsi tse kang melanoma, kankere ea mala, kankere ea qoqotho le kankere ea matšoafo.Ho utloisisa phetoho ea liphatsa tsa lefutso tsa BRAF e se e le tlhoko ea ho hlahloba li-EGFR-TKIs le litlhare tse lebisitsoeng ho liphatsa tsa lefutso tsa BRAF kalafong ea meriana e lebisitsoeng ho bakuli ba ka ruang molemo.
Channel
| FAM | Phetoho ea V600E, taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | disampole tsa dithishu tsa pathological tse kentsweng ke parafine |
| CV | 5.0% |
| Ct | ≤38 |
| LoD | Sebelisa lisebelisoa ho bona taolo e tsamaellanang ea boleng ba LoD.a) tlas'a 3ng/μL semelo sa mofuta o hlaha, sekhahla sa phetoho ea 1% se ka bonoa ka har'a buffer ea karabelo e tsitsitseng;b) tlas'a sekhahla sa phetoho ea 1%, phetoho ea 1 × 103Likopi/mL ka mokokotlong oa mofuta o hlaha oa 1×105Likopi/mL li ka bonoa ka mokhoa o tsitsitseng ka har'a buffer ea karabelo;c) IC Reaction Buffer e ka bona moeli o tlase oa taolo ea boleng ba SW3 ea taolo ea kahare ea khamphani. |
| Lisebelisoa tse Sebetsang: | Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7300 Real-Nako PCR Systems, QuantStudio® 5 Real-Time PCR Systems Sesebelisoa sa PCR sa LightCycler®480 Real-Time Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 |
Phallo ea Mosebetsi
Lisebelisuoa tse khothalelitsoeng: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Parafini-embedded Tissue DNA Rapid Extraction Kit (DP330) e entsoeng ke Tiangen Biotech(Beijing) Co., Ltd.
