Motho CYP2C9 le VKORC1 Gene Polymorphism
Lebitso la sehlahisoa
HWTS-GE014A-Human CYP2C9 le VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Warfarin ke anticoagulant ea molomo e sebelisoang hangata ts'ebetsong ea bongaka hona joale, e reretsoeng haholo ho thibela le ho phekola mafu a thromboembolic.Leha ho le joalo, warfarin e na le fensetere e fokolang ea phekolo 'me e fapana haholo har'a merabe le batho ka bomong.Lipalo-palo li bontšitse hore phapang ea tekanyo e tsitsitseng ho batho ba fapaneng e ka ba makhetlo a fetang 20.Karabelo e mpe ea mali e hlaha ho 15.2% ea bakuli ba noang warfarin selemo se seng le se seng, bao 3.5% ea bona ba tsoa mali a bolaeang.Lithuto tsa pharmacogenomic li bonts'itse hore genetic polymorphism ea target enzyme VKORC1 le metabolic enzyme CYP2C9 ea warfarin ke ntlha ea bohlokoa e amang phapang ea lethal dose ea warfarin.Warfarin ke inhibitor e khethehileng ea vithamine K epoxide reductase (VKORC1), 'me kahoo e thibela motsoako oa lintho tse thibelang mali tse amanang le vithamine K' me e fana ka anticoagulation.Palo e kholo ea liphuputso e bonts'itse hore mofuta oa polymorphism oa motšehetsi oa VKORC1 ke eona ntho ea bohlokoahali e amang peiso le liphapang tsa motho ka mong ho lethal dose le hlokahalang la warfarin.Warfarin e entsoe ka CYP2C9, 'me liphetoho tsa eona li fokotsa metabolism ea warfarin butle haholo.Batho ba sebelisang warfarin ba na le kotsi e kholo (habeli ho isa ho makhetlo a mararo) ea ho tsoa mali qalong ea tšebeliso.
Channel
| FAM | VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Madi a macha a anticoagulated a EDTA |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Tse khethehileng | Ha ho na cross-reactivity le tatellano e 'ngoe e tsitsitseng haholo ea genome ea motho (motho CYP2C19 gene, RPN2 ea motho);phetoho ea CYP2C9*13 le VKORC1 (3730G>A) ka ntle ho sebaka sa ho lemoha |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).
