Human Papillomavirus (Mefuta e 28) Genotyping Detection Kit (Fluorescence PCR)
Lebitso la Sehlahiswa
HWTS-CC004A-Human Papillomavirus (Mefuta e 28) Genotyping Detection Kit (Fluorescence PCR)
Epidemiology
Kit e sebelisa mokhoa oa ho lemoha oa "nucleic acid amplification" (PCR) fluorescence.Li-primers le li-probes tse ikhethileng li entsoe ho ipapisitse le letoto la liphatsa tsa lefutso tsa L1 tsa HPV.Probe e khethehileng e ngotsoe ka FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore, HP435 , 45, 54, 56, 68, 82) le ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) ho 5', 'me sehlopha sa 3' quencher ke BHQ1 kapa BHQ2.Nakong ea ntlafatso ea PCR, li-primers le li-probes li tlamella tatellano ea tsona e fapaneng.Ha enzyme ea Taq e kopana le li-probes tse tlamahaneng le tatellano ea sepheo, e etsa mosebetsi oa 5 'end exonuclease ho arola motlalehi oa fluorophore ho quencher fluorophore, e le hore tsamaiso ea fluorescence monitoring e ka fumana letšoao la fluorescent, ke hore, nako le nako ha DNA. strand e matlafatsoa, molek'hule ea fluorescent e thehoa, e hlokomelang tumellano e feletseng ea ho bokelloa ha matšoao a fluorescent le ho thehoa ha lihlahisoa tsa PCR, e le ho finyella ho fumanoa ha boleng le genotyping ea nucleic acid ea mefuta e 28 ea papillomavirus ea motho ka lisampole tsa lisele tsa cervical exfoliated. .
Channel
| FAM | 16,58,53,73,6,26,40. |
| VIC/HEX | 18,33,51,59,11,81,43 |
| ROX | 31,66,52,39,83,61,42 |
| CY5 | 56,35,45,68,54,44,82 |
Tekheniki Parameters
| Polokelo | ≤-18℃ Lefifing |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Lisele tsa cervical exfoliated |
| Ct | ≤25 |
| CV | ≤5.0% |
| LoD | 25 likopi/karabelo |
| Lisebelisoa tse sebetsang | Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600)
Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems
QuantStudio®5 Nako ea 'Nete ea PCR Systems
Litsamaiso tsa PCR tsa nako ea 'nete tsa SLAN-96P
Sesebelisoa sa PCR sa LightCycler®480 Real-Time
LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi
MA-6000 Real-Nako Quantitative Thermal Cycler
Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96
BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).
Khetho ea 2.
Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
