KRAS 8 Liphetoho
Lebitso la sehlahisoa
HWTS-TM014-KRAS 8 Phetoho ea ho Hlahloba Kit (Fluorescence PCR)
HWTS-TM011-Omisitsoeng-KRAS 8 Mutations Detection Kit(Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Liphetoho tsa mofuta oa lefutso la KRAS li fumanoe mefuteng e mengata ea lihlahala tsa batho, hoo e ka bang 17% ~ 25% sekhahla sa phetoho ho hlahala, 15% ~ 30% sekhahla sa phetoho ho bakuli ba mofetše oa matšoafo, 20% ~ 50% sekhahla sa phetoho mofetšeng oa mala. bakuli.Hobane protheine ea P21 e kentsoeng ke mofuta oa K-ras e fumaneha ka tlase ho tsela ea EGFR ea pontšo, ka mor'a phetoho ea liphatsa tsa lefutso tsa K-ras, tsela ea pontšo e tlaase e lula e sebetsa 'me ha e amehe ke lithethefatsi tse lebisitsoeng holimo ho EGFR, e leng se bakang ho tsoela pele. ho ata ho kotsi ha lisele.Liphetoho tsa lefutso la K-ras hangata li fana ka khanyetso ho EGFR tyrosine kinase inhibitors ho bakuli ba mofetše oa matšoafo le ho hanyetsa lithethefatsi tsa anti-EGFR ho bakuli ba mofetše oa colorectal.Ka 2008, National Comprehensive Cancer Network (NCCN) e ile ea fana ka tataiso ea kliniki bakeng sa mofetše oa colorectal, e ileng ea bontša hore libaka tsa phetoho tse etsang hore K-ras li sebetse li fumaneha haholo ka li-codon 12 le 13 tsa exon 2, 'me e khothalelitse hore bakuli bohle ba nang le kankere e tsoetseng pele ea metastatic colorectal ba ka hlahlojoa phetoho ea K-ras pele ba phekoloa.Ka hona, ho lemoha ka potlako le ka nepo phetoho ea liphatsa tsa lefutso tsa K-ras ho bohlokoa haholo tataisong ea meriana ea bongaka.Setsi sena se sebelisa DNA e le sampole ea ho lemoha ho fana ka tlhahlobo ea boleng ba boemo ba phetoho, e ka thusang baoki ho hlahloba mofetše oa mala, mofetše oa matšoafo le bakuli ba bang ba hlahala ba ruang molemo ho litlhare tse lebisitsoeng.Liphetho tsa liteko tsa kit ke tsa litšupiso tsa bongaka feela 'me ha lia lokela ho sebelisoa e le eona feela motheo oa kalafo e ikhethileng ea bakuli.Baoki ba lokela ho etsa likahlolo tse felletseng mabapi le liphetho tsa liteko tse ipapisitseng le lintlha tse kang boemo ba mokuli, matšoao a lithethefatsi, karabelo ea kalafo le matšoao a mang a tlhahlobo ea laboratori.
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ Lefifing;Lyophilized: ≤30℃ Lefifing |
| Shelf-bophelo | Mokelikeli: likhoeli tse 9;Lyophilized: likhoeli tse 12 |
| Mofuta oa Mohlala | parafine e kentsoeng ka har'a lisele tsa pathological kapa karolo e na le lisele tsa tumor |
| CV | ≤5.0% |
| LoD | K-ras Reaction Buffer A le K-ras Reaction Buffer B e ka bona ka mokhoa o tsitsitseng 1% sekhahla sa phetoho tlas'a 3ng/μL mokokotlo oa mofuta o hlaha |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7300 Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems Sistimi ea PCR ea LightCycler® 480 ea Nako ea 'Nete Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 |
Phallo ea Mosebetsi
E khothaletsoa ho sebelisa QIAGEN's QIAamp DNA FFPE Tissue Kit (56404) le Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) e entsoeng ke Tiangen Biotech(Beijing) Co., Ltd.
