Mycobacterium Tuberculosis INH Resistance
Lebitso la Sehlahiswa
HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)
Epidemiology
Isoniazid, sethethefatsi se ka sehloohong se thibelang lefuba se hlahisitsoeng ka 1952, ke e 'ngoe ea meriana e sebetsang ka ho fetisisa bakeng sa phekolo e kopantsoeng ea lefuba le sebetsang le moriana o le mong oa lefuba le ipatileng.
KatG ke mofuta o ka sehloohong o kenyang catalase-peroxidase mme phetoho ea liphatsa tsa lefutso ea katG e ka khothaletsa motsoako oa lebota la lisele tsa mycolic acid, ho etsa hore baktheria e hanyetse isoniazid.Polelo ea KatG e amahanngoa hampe le liphetoho ho INH-MIC, 'me ho fokotseha ha 2 ho polelo ea katG ho fella ka keketseho e nyenyane ea 2 ho MIC.Lebaka le leng la ho hanyetsa isoniazid ho lefuba la mycobacterium le etsahala ha ho kenngoa ha motheo, ho tlosoa kapa ho fetoha ho etsahala sebakeng sa InhA sa lefuba la mycobacterium lefuba.
Channel
| ROX | inhA (-15C>T) sebaka· |
| CY5 | sebaka sa katG (315G>C). |
| VIC (HEX) | IS6110 |
Tekheniki Parameters
| Polokelo | ≤-18℃ Lefifing |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Sekhohlela |
| CV | ≤5.0% |
| LoD | 1 × 103libaktheria/mL |
| Tse khethehileng | No-cross reactivity le liphetoho tsa libaka tse 'nè tsa ho hanyetsa lithethefatsi (511, 516, 526 le 531) tsa gene ea rpoB ka ntle ho sebaka sa ho lemoha ha lisebelisoa tsa ho lemoha. Lisebelisoa tse Sebetsang: Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
