Mycobacterium Tuberculosis Rifampicin Resistance
Lebitso la sehlahisoa
HWTS-RT074A-Mycobacterium Tuberculosis Rifampicin Resistance Detection Kit (Fluorescence PCR)
Epidemiology
Rifampicin esale e sebelisoa haholo kalafong ea bakuli ba lefuba la matšoafo ho tloha bofelong ba lilemo tsa bo-1970, 'me e na le phello e kholo.E bile khetho ea pele ea ho khutsufatsa chemotherapy ea bakuli ba lefuba la matšoafo.Ho hanyetsa ha Rifampicin ho bakoa haholo-holo ke phetoho ea mofuta oa rpoB.Le hoja litlhare tse ncha tse thibelang lefuba li lula li tsoa, 'me katleho ea bongaka ea bakuli ba lefuba la matšoafo le eona e tsoetse pele ho ntlafala, ho ntse ho e-na le khaello e itseng ea lithethefatsi tse thibelang lefuba,' me ketsahalo ea tšebeliso e sa utloahaleng ea tšebeliso ea lithethefatsi litleleniki e batla e phahame.Ho hlakile hore lefuba la Mycobacterium ho bakuli ba nang le lefuba la matšoafo le ke ke la bolaoa ka ho feletseng ka nako e loketseng, e leng se qetellang se lebisa likarolong tse fapaneng tsa ho hanyetsa lithethefatsi 'meleng oa mokuli, ho lelefatsa nako ea lefu lena, le ho eketsa kotsi ea lefu la mokuli.Setsi sena se loketse tlhahlobo ea tlatsetso ea tšoaetso ea lefuba la Mycobacterium le ho lemoha lefutso la rifampicin, e leng ho thusang ho utloisisa khanyetso ea meriana ea lefuba la mycobacterium le tšoaelitsoeng ke bakuli, le ho fana ka mekhoa e thusang bakeng sa tataiso ea meriana ea bongaka.
Tekheniki Parameters
Polokelo | ≤-18℃ Lefifing |
Shelf-bophelo | Likhoeli tse 9 |
Mofuta oa Mohlala | Sekhohlela |
CV | ≤5.0% |
LoD | mofuta oa hlaha o hananang le rifampicin: 2x103libaktheria/mL phetoho ea homozygous: 2x103libaktheria/mL |
Tse khethehileng | Setsi sena ha se na kamano le lefutso la motho, likokoana-hloko tse ling tse se nang lefuba, le likokoana-hloko tsa pneumonia.E lemoha libaka tsa phetoho ea liphatsa tsa lefutso tse hanyetsanang le lithethefatsi tsa mofuta oa hlaha oa mycobacterium lefuba tse kang katG 315G>C\A, InhA-15C> T, liphetho tsa liteko li bontša hore ha ho na khanyetso ho rifampicin, ho bolelang hore ha ho na karabelo e fapaneng. |
Lisebelisoa tse Sebetsang: | Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P |
Phallo ea Mosebetsi
Khetho ea 1.
Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48) le Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).
Khetho ea 2.
Sehlahisoa se khothalelitsoeng sa ho ntšoa: Setsi sa Nucleic Acid kapa Purification Reagent(YDP302) ke Tiangen Biotech(Beijing) Co.,Ltd.