Mycobacterium Tuberculosis Nucleic Acid le Rifampicin Resistance

Tlhaloso e Khutšoanyane:

Setsi sena se loketse tlhahlobo ea boleng ba Mycobacterium tuberculosis DNA disampole tsa sekhohlela sa motho in vitro, hammoho le phetoho ea homozygous sebakeng sa 507-533 amino acid codon ea mofuta oa rpoB e bakang Mycobacterium tuberculosis rifampicin resistance.

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HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid le Rifampicin Resistance Detection Kit (Melting Curve)

Setifikeiti

Epidemiology

Mycobacterium tuberculosis , haufinyane joalo ka Tubercle bacillus, TB, ke baktheria ea pathogenic e bakang lefuba.Hajoale, litlhare tse thibelang lefuba tse sebelisoang hangata tsa moleng oa pele li kenyelletsa isoniazid, rifampicin le hexambutol, joalo-joalo. Litlhare tsa mofuta oa bobeli tse thibelang lefuba li kenyelletsa fluoroquinolones, amikacin le kanamycin, joalo-joalo. Litlhare tse ncha tse ntlafalitsoeng ke linezolid, bedaquiline le delamani, joalo-joalo. Leha ho le joalo, ka lebaka la tšebeliso e fosahetseng ea lithethefatsi tse thibelang lefuba le litšobotsi tsa sebopeho sa lerako la sele ea lefuba la mycobacterium, lefuba la mycobacterium le hlahisa ho hanyetsa lithethefatsi khahlanong le lithethefatsi tse thibelang lefuba, tse tlisang mathata a tebileng ho thibela le ho phekola lefuba.

Rifampicin esale e sebelisoa haholo kalafong ea bakuli ba lefuba la matšoafo ho tloha bofelong ba lilemo tsa bo-1970, 'me e na le phello e kholo.E bile khetho ea pele ea ho khutsufatsa chemotherapy ea bakuli ba lefuba la matšoafo.Ho hanyetsa ha Rifampicin ho bakoa haholo-holo ke phetoho ea mofuta oa rpoB.Le hoja litlhare tse ncha tse thibelang lefuba li lula li tsoa, 'me katleho ea bongaka ea bakuli ba lefuba la matšoafo le eona e tsoetse pele ho ntlafala, ho ntse ho e-na le khaello e itseng ea lithethefatsi tse thibelang lefuba,' me ketsahalo ea tšebeliso e sa utloahaleng ea tšebeliso ea lithethefatsi litleleniki e batla e phahame.Ho hlakile hore lefuba la Mycobacterium ho bakuli ba nang le lefuba la matšoafo le ke ke la bolaoa ka ho feletseng ka nako e loketseng, e leng se qetellang se lebisa likarolong tse fapaneng tsa ho hanyetsa lithethefatsi 'meleng oa mokuli, ho lelefatsa nako ea lefu lena, le ho eketsa kotsi ea lefu la mokuli.

Channel

Channel	Likanale le Fluorophores	Reaction Buffer A	Reaction Buffer B	Reaction Buffer C
Mokhatlo oa FAM	Motlalehi: FAM, Quencher: Ha ho letho	rpoB 507-514	rpoB 513-520	38KD le IS6110
CY5 Channel	Motlalehi: CY5, Quencher: Ha ho letho	rpoB 520-527	rpoB 527-533	/
HEX (VIC) Channel	Motlalehi: HEX (VIC), Quencher: Ha ho letho	Taolo ya ka hare	Taolo ya ka hare	Taolo ya ka hare

Tekheniki Parameters

Polokelo	≤-18℃ Lefifing
Shelf-bophelo	Likhoeli tse 12
Mofuta oa Mohlala	Sekhohlela
CV	≤5.0%
LoD	lefuba la mycobacterium 50 baktheria/mL mofuta oa hlaha o hananang le rifampicin: 2x10³libaktheria/mL phetoho ea homozygous: 2x10³libaktheria/mL
Tse khethehileng	E lemoha mofuta oa hlaha oa lefuba la mycobacterium le libaka tsa phetoho ea liphatsa tsa lefutso tse hanyetsanang le lithethefatsi tse kang katG 315G>C\A, InhA-15C> T, liphetho tsa liteko li bontša hore ha ho na khanyetso ho rifampicin, ho bolelang hore ha ho na cross-reactivity.
Lisebelisoa tse Sebetsang:	Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 LightCycler480® Real-Time PCR System