Staphylococcus Aureus le Methicillin-Resistant Staphylococcus Aureus Nucleic Acid
Lebitso la sehlahisoa
HWTS-OT062-Staphylococcus Aureus le Methicillin-Resistant Staphylococcus Aureus Nucleic Acid Detection Kit (Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Staphylococcus aureus ke e 'ngoe ea libaktheria tsa bohlokoa tsa pathogenic tsa nosocomial.Staphylococcus aureus (SA) ke ea staphylococcus 'me ke moemeli oa baktheria ea Gram-positive, e ka hlahisang mefuta e mengata ea chefo le li-enzyme tse hlaselang.Libaktheria li na le litšobotsi tsa ho ajoa ka bophara, pathogenicity e matla le sekhahla se phahameng sa ho hanyetsa.Thermostable nuclease gene (nuc) ke mofuta o bolokehileng haholo oa staphylococcus aureus.Lilemong tsa morao tjena, ka lebaka la tšebeliso e pharaletseng ea li-hormone le litokisetso tsa 'mele oa ho itšireletsa mafung le tlhekefetso ea lithibela-mafu tse pharaletseng, mafu a nosocomial a bakoang ke Staphylococcus aureus (MRSA) e hanyetsanang le Methicillin ho Staphylococcus a ntse a eketseha.Palo ea kakaretso ea naha ea MRSA e ne e le 30.2% ka 2019 Chaena.MRSA e arotsoe ka MRSA e amanang le tlhokomelo ea bophelo (HA-MRSA), MRSA e amanang le sechaba (CA-MRSA), le MRSA e amanang le mehlape (LA-MRSA).CA-MRSA, HA-MRSA, LA-MRSA e na le phapang e kholo ho microbiology, ho hanyetsa baktheria (mohlala, HA-MRSA e bontša ho hanyetsa lithethefatsi tse ngata ho feta CA-MRSA) le litšobotsi tsa kliniki (mohlala, sebaka sa tšoaetso).Ho ea ka litšobotsi tsena, CA-MRSA le HA-MRSA li ka khetholloa.Leha ho le joalo, phapang pakeng tsa CA-MRSA le HA-MRSA e ntse e fokotseha ka lebaka la motsamao o sa khaotseng oa batho pakeng tsa lipetlele le sechaba.MRSA e hanyetsana le meriana e mengata, ha e hanyetsane le lithibela-mafu tsa β-lactam feela, empa hape le ho aminoglycosides, macrolides, tetracyclines le quinolones ka tekanyo e fapaneng.Ho na le liphapang tse kholo tsa libaka tsa litekanyetso tsa ho hanyetsa lithethefatsi le mekhoa e fapaneng.
Methicillin resistance mecA gene e bapala karolo ea makhaola-khang khahlanong le staphylococcal.Mofuta ona o jara ntho e ikhethang ea liphatsa tsa lefutso (SCCmec), e kenyelletsang protheine e tlamang penicillin 2a (PBP2a) mme e na le kamano e tlase le lithibela-mafu tsa β-lactam, e le hore litlhare tse thibelang likokoana-hloko li se ke tsa sitisa ho qaptjoa ha lera la peptidoglycan ea sele, se bakang kganetso ya dithethefatsi.
Channel
| FAM | Mofuta oa lefutso oa mecA o hananang le methicillin |
| CY5 | staphylococcus aureus nuc gene |
| VIC/HEX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | sekhohlela, lisampole tsa tšoaetso ea letlalo le lisele tse bonolo, le lisampole tse felletseng tsa mali |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL |
| Tse khethehileng | Ha ho na cross-reactivity le likokoana-hloko tse ling tsa phefumoloho tse kang methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsicinespneumoniaenteritis, klebsimanobanilla pneumoniae, klebsimantobaniella pneumoniaeter, acerococcus epidermidis. kokoana-hloko, streptococcus pneumoniae , enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
