Staphylococcus Aureus le Methicillin-Resistant Staphylococcus Aureus
Lebitso la sehlahisoa
HWTS-OT062 Staphylococcus Aureus le Methicillin-Resistant Staphylococcus Aureus Nucleic Acid Detection Kit(Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Staphylococcus aureus ke e 'ngoe ea libaktheria tsa bohlokoa tsa pathogenic tsa nosocomial.Staphylococcus aureus (SA) ke ea staphylococcus 'me ke moemeli oa baktheria ea Gram-positive, e ka hlahisang mefuta e mengata ea chefo le li-enzyme tse hlaselang.Libaktheria li na le litšobotsi tsa ho ajoa ka bophara, pathogenicity e matla le sekhahla se phahameng sa ho hanyetsa.Thermostable nuclease gene (nuc) ke mofuta o bolokehileng haholo oa staphylococcus aureus.
Channel
| FAM | Mofuta oa lefutso oa mecA o hananang le methicillin |
| ROX | Taolo ea ka hare |
| CY5 | staphylococcus aureus nuc gene |
Tekheniki Parameters
| Polokelo | ≤-18℃ & e sirelelitsoe khanyeng |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | sekhohlela, disampole tsa tshwaetso ya letlalo le dithishu tse bonolo, le disampole tsa swab ya nko |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL libaktheria tse hanyetsanang le methicillin.Ha khiti e lemoha tšupiso ea naha ea LoD, 1000/mL staphylococcus aureus e ka bonoa. |
| Tse khethehileng | Teko ea cross-reactivity e bonts'a hore kit ena ha e na reactivity ea sefapano le likokoana-hloko tse ling tsa phefumoloho tse kang methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, pneumonialitococcus, pneumoniaebacoccus, bacerichicoccus, basculitis, pneumoniae, coagulase, aeruginosa. rona mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. e ka sebelisoa le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Eketsa 200µL ea saline e tloaelehileng mocheng o sebelitsoeng, 'me mehato e latelang e lokela ho ntšoa ho latela litaelo,' me bophahamo ba molumo o khothaletsoang ke 80µL.
Khetho ea 2.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kenya 1mL ea saline e tloaelehileng ho metsi ka mor'a ho hlatsoa ka saline e tloaelehileng, ebe u kopanya hantle.Centrifuge ho 13,000r/min bakeng sa metsotso e 5, tlosa supernatant (boloka 10-20µL ea supernatant), 'me u latele litaelo bakeng sa ho ntšoa ho latelang.
Sehlahisoa se khothalelitsoeng sa ho ntšoa: Nucleic Acid Extraction kapa Purification Reagent (YDP302) ke Tiangen Biotech (Beijing) Co.,Ltd.Ho ntšoa ho lokela ho etsoa ka tieo ho latela mohato oa 2 oa bukana ea litaelo.Ho khothaletsoa ho sebelisa metsi a se nang RNase le a DNase bakeng sa tlhahiso ea molumo oa 100µL.
