Motho CYP2C19 Gene Polymorphism

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho lemoha boleng ba in vitro polymorphism ea liphatsa tsa lefutso tsa CYP2C19 CYP2C19 * 2 (rs4244285, c.681G> A), CYP2C19 * 3 (rs4986893, c.636G> A), CYP2C19 * 16 c680 (rs4986893) > T) ho DNA ea genomic ea lisampole tsa mali a motho kaofela.


Lintlha tsa Sehlahisoa

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Lebitso la sehlahisoa

HWTS-GE012A-Human CYP2C19 Gene Polymorphism Detection Kit (Fluorescence PCR)

Setifikeiti

CE

Epidemiology

CYP2C19 ke e 'ngoe ea li-enzyme tsa bohlokoa tse hlahisang li-enzyme lelapeng la CYP450.Li-substrates tse ngata tsa endogenous le hoo e ka bang 2% ea litlhare tsa kliniki li sebelisoa ke CYP2C19, joalo ka metabolism ea antiplatelet aggregation inhibitors (joalo ka clopidogrel), proton pump inhibitors (omeprazole), anticonvulsants, joalo-joalo. lithethefatsi tse amanang.Liphetoho tsena tsa lintlha tsa *2 (rs4244285) le *3 (rs4986893) li baka tahlehelo ea ts'ebetso ea enzyme e kentsoeng ke lefutso la CYP2C19 le bofokoli ba bokhoni ba metabolic substrate, le ho eketsa khatello ea mali, e le ho baka litlamorao tse mpe tsa lithethefatsi tse amanang le khatello ea mali.*17 (rs12248560) e ka eketsa ts'ebetso ea enzyme e kentsoeng ke CYP2C19 gene, tlhahiso ea metabolites e sebetsang, le ho ntlafatsa thibelo ea ho bokellana ha platelet le ho eketsa kotsi ea ho tsoa mali.Bakeng sa batho ba nang le metabolism e liehang ea lithethefatsi, ho noa litekanyetso tse tloaelehileng ka nako e telele ho tla baka chefo e tebileng le litla-morao: haholo-holo tšenyo ea sebete, tšenyo ea tsamaiso ea hematopoietic, tšenyo ea tsamaiso ea methapo ea bohareng, joalo-joalo, e ka lebisang lefung maemong a boima.Ho ea ka liphapang tsa motho ka mong ho metabolism e tsamaellanang le lithethefatsi, ka kakaretso e arotsoe ka li-phenotypes tse 'ne, e leng ultra-fast metabolism (UM,*17/*17,*1/*17), metabolism e potlakileng (RM,*1/*1) ), metabolism e bohareng (IM, *1/*2, *1/*3), metabolism e liehang (PM, *2/*2, *2/*3, *3/*3).

Channel

FAM CYP2C19*2
CY5 CYP2C9*3
ROX CYP2C19*17
VIC/HEX IC

Tekheniki Parameters

Polokelo Mokelikeli: ≤-18℃
Shelf-bophelo Likhoeli tse 12
Mofuta oa Mohlala Madi a macha a anticoagulated a EDTA
CV ≤5.0%
LoD 1.0ng/μL
Tse khethehileng Ha ho na cross-reactivity le tatellano e meng e tsitsitseng haholo (CYP2C9 gene) ho genome ea motho.Liphetoho tsa libaka tsa CYP2C19 * 23, CYP2C19 * 24 le CYP2C19 * 25 tse ka ntle ho sebaka sa ho lemoha ha setlolo sena ha li na phello holima phello ea ho lemoha ha sesebelisoa sena.
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Phallo ea Mosebetsi

Sehlahisoa sa ho ntša se khothalelitsoeng:Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).


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