Methylated ea Motho NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene

Tlhaloso e Khutšoanyane:

Kit e reretsoe ho lemoha boleng ba in vitro ea liphatsa tsa lefutso tsa methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 ka liseleng tsa mala a exfoliated ka lisampole tsa setulo sa motho.


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene Detection Kit (Fluorescence PCR)

Setifikeiti

CE

Epidemiology

Ho batho ba baholo, lisele tse fetang 10 8 tsa mala epithelial li oela leboteng la mala letsatsi le leng le le leng, 'me li ntšoa ka mantle ka peristalsis e kholo ea mala.Ka lebaka la lisele tsa hlahala li ka 'na tsa oela ka har'a mala a ho ata ho sa tloaelehang, setulo sa bakuli ba mala se na le lisele tse ngata tse kulang le likarolo tse sa tloaelehang tsa lisele, e leng motheo oa thepa bakeng sa ho lemoha mantle a tsitsitseng.Liphuputso li fumane hore phetoho ea methylation ea bahlahisi ba liphatsa tsa lefutso ke ketsahalo ea pele ea tumorigenesis, 'me lisebelisoa tsa liphatsa tsa lefutso tse fumanoang ho tloha lisampole tsa batho ba nang le mofetše oa colorectal li ka bontša boteng ba kankere ka maleng pejana.

NDRG4, e tsejoang hape e le SMAP-8 le BDM1, ke e 'ngoe ea litho tse' nè tsa NDRG gene family (NDRG1-4), e bontšitsoeng hore e amahanngoa le ho ata ha lisele, ho arohana, tsoelo-pele le khatello ea kelello.Ho netefalitsoe hore NDRG4 methylation ke biomarker e ka bang teng bakeng sa tlhahlobo e sa hlaseheng ea mofetše oa colorectal ka lisampole tsa mantle.

SEPT9 ke setho sa lelapa la lefutso la Septin, le nang le bonyane liphatsa tsa lefutso tse 13 tse kenyelletsang sebaka se bolokiloeng sa GTPase se ka kopanyang liprotheine tse amanang le cytoskeleton, 'me e amahanngoa le karohano ea lisele le tumorigenesis.Boithuto bo fumane hore litaba tsa methylated Septin9 tsa liphatsa tsa lefutso li eketsoa lisampole tsa mantle ho tsoa ho bakuli ba nang le mofetše oa colorectal.

Liprotheine tse amanang le frizzled-related frizzled (sFRPs) ke liprotheine tse qhibilihang tseo e leng sehlopha sa bahanyetsi ba Wnt pathway ka lebaka la sebopeho sa bona se phahameng sa homology ho frizzled (Fz) receptor bakeng sa pontšo ea Wnt.Ho se sebetse ha mofuta oa SFRP ho fella ka ts'ebetso e sa laoleheng ea pontšo ea Wnt e amanang le mofetše oa colorectal.Hajoale, SFRP2 methylation ka setulong e ka sebelisoa e le biomarker e sa hlaseleng bakeng sa tlhahlobo ea mofets'e oa colorectal.

BMP3 ke setho sa TGF-B superfamily mme ka hona e bapala karolo ea bohlokoa ho nts'etsopele ea embryonic ka ho susumetsa le ho bopa popeho ea pele ea masapo.BMP3 ke hypermethylated ka mofetše oa mala 'me e ka sebelisoa e le letšoao la bohlokoa la hlahala.

SDC2 ke cell surface heparan sulfate proteoglycan e amehang taolong ea mekhoa e mengata ea fisioloji le ea mafu.Ho sebetsa 'meleng ho kenyelletsa ho ata ha lisele, ho arohana, ho khomarela, mokhatlo oa cytoskeletal, ho falla, ho folisa maqeba, puisano ea lisele-matrix, angiogenesis;Mekhoa ea pathological e kenyelletsa ho ruruha le kankere.Boemo ba methylation ba lefutso la SDC2 liseleng tsa mofetše oa colorectal bo ne bo phahame haholo ho feta ba lisele tse tloaelehileng.

Channel

Tšireletso ea karabelo A

VIC/HEX mofuta oa methylated NDRG4
ROX mofuta oa methylated SEPT9
CY5 taolo ya ka hare

Tšireletso ea karabelo B

VIC/HEX mofuta oa methylated SFRP2
ROX mofuta oa methylated BMP3
FAM mofuta oa methylated SDC2
CY5 taolo ya ka hare

Tlhaloso

Gene

Letshwao la Letshwao

Ct Boleng

Tlhaloso

NDRG4

VIC (HEX)

Ct boleng≤38

NDRG4 e ntle

Ct value>38 kapa unde

NDRG4 e mpe

SEPT9

ROX

Ct boleng≤38

SEPT9 e ntle

Ct value>38 kapa unde

SEPT9 e mpe

SFRP2

VIC (HEX)

Ct boleng≤38

SFRP2 e ntle

Ct value>38 kapa unde

SFRP2 e mpe

Tekheniki Parameters

Polokelo Mokelikeli: ≤-18℃
Shelf-bophelo Likhoeli tse 9
Mofuta oa Mohlala Mohlala oa setulo
CV ≤5.0%
Tse khethehileng Ha ho na cross-reactivity le mofets'e oa sebete, mofets'e oa bile, mofets'e oa qoqotho le mofets'e oa matšoafo
Lisebelisoa tse sebetsang QuantStudio ®5 Nako ea 'Nete ea PCR Systems

Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P

Sesebelisoa sa PCR sa LightCycler®480 Real-Time

LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi

MA-6000 Real-Nako Quantitative Thermal Cycler

Phallo ea Mosebetsi

Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).


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