Mycobacterium Tuberculosis Isoniazid Resistance Phetoho

Mycobacterium Tuberculosis Isoniazid Resistance Mutation Featured Image

Tlhaloso e Khutšoanyane:

Setsi sena se loketse ho lemoha boleng ba libaka tse ka sehloohong tsa phetoho ea li-sputum tsa batho tse bokelitsoeng ho bakuli ba Tubercle bacillus positive tse lebisang ho mycobacterium lefuba la isoniazid resistance: InhA promoter region -15C>T, -8T>A, -8T>C;Sebaka sa papatso sa AhpC -12C>T, -6G>A;phetoho ea homozygous ea KatG 315 codon 315G>A, 315G>C.

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HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Detection Kit (Melting Curve)

Epidemiology

Mycobacterium tuberculosis, haufinyane joalo ka Tubercle bacillus (TB), ke baktheria ea pathogenic e bakang lefuba.Hajoale, litlhare tse thibelang lefuba tse sebelisoang hangata tsa moleng oa pele li kenyelletsa isoniazid, rifampicin le hexambutol, joalo-joalo. Litlhare tsa mofuta oa bobeli tse thibelang lefuba li kenyelletsa fluoroquinolones, amikacin le kanamycin, joalo-joalo. Litlhare tse ncha tse ntlafalitsoeng ke linezolid, bedaquiline le delamani, joalo-joalo. Leha ho le joalo, ka lebaka la tšebeliso e fosahetseng ea lithethefatsi tse thibelang lefuba le litšobotsi tsa sebopeho sa lerako la sele ea lefuba la mycobacterium, lefuba la mycobacterium le hlahisa ho hanyetsa lithethefatsi khahlanong le lithethefatsi tse thibelang lefuba, tse tlisang mathata a tebileng ho thibela le ho phekola lefuba.

Channel

FAM	MP nucleic acid
ROX	Taolo ea ka hare

Tekheniki Parameters

Polokelo	≤-18℃
Shelf-bophelo	Likhoeli tse 12
Mofuta oa Mohlala	sekhohlela
CV	≤5.0%
LoD	Moeli oa ho lemoha bakeng sa libaktheria tse hanyetsanang tsa mofuta o hlaha oa isoniazid ke 2x103 bacteria/mL, 'me moeli oa ho lemoha ha baktheria e fetohang ke 2x103 bacteria/mL.
Tse khethehileng	a.Ha ho na karabelo e fapaneng har'a genome ea motho, likokoana-hloko tse ling tsa mycobacteria le pneumonia tse fumanoeng ke setsi sena. b.Libaka tsa phetoho ea liphatsa tsa lefutso tse hanyetsanang le lithethefatsi tse hlaha ho mofuta oa hlaha oa Mycobacterium lefuba, joalo ka sebaka se khethollang khanyetso ea mofuta oa rifampicin rpoB, li ile tsa bonoa, 'me liphetho tsa liteko li bonts'itse ha ho na khanyetso ho isoniazid, tse bonts'ang hore ha ho ts'ebetso e fapaneng.
Lisebelisoa tse sebetsang	Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P BioRad CFX96 Real-Nako PCR Systems LightCycler480® Real-Time PCR System

Phallo ea Mosebetsi

Haeba u sebelisa Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test. Med-Tech Co., Ltd. bakeng sa ho ntšoa, eketsa 200μL ea taolo e mpe le sampole ea sekhohlela e sebelitsoeng ho hlahlojoa ka tatellano, ebe o eketsa 10μL ea taolo ea kahare ka thoko ho taolo e mpe, sampole ea sekhohlela se sebelitsoeng ho hlahlojoa, 'me mehato e latelang e lokela ho etsoa ka thata ho latela litaelo tsa ho ntšoa.Bolumo ea sampole e ntšitsoeng ke 200μL, 'me bophahamo ba modumo e khothaletsoang ke 100μL.